Assay Of Sulphamethoxazole

 

Aim: To determine Percentage purity of sulphamethoxazole.

Apparatus: 

• Erlenmeyer flask
• Volumetric flask
• Pipette
•  Burette

Chemicals Required: 

• Sulphametoxazole
• Sulphanilic acid
• Sodium nitrate
• Potassium bromide
• Hydrochloricacid 
• Starch iodide external indiacator.

Principle: The principle involved in the assay of Sulphamethoxazole is diazotization where the sodium nitrite consumed by sulphamethoxazole to from diazonium salt is calculated by using starch iodide as an external indicator which gives blue color as an end point.

Procedure: 

    Standardization of 0.1M NaNO₂ :

Weigh about 0.3gm of sulphanilic acid and dissolve in 50ml of 2M hcL. Add 3gm of potassium bromide, cool in ice and titrate with 0.1M NaNO₂ using strach iodide as an indicator. Repeat the titration with blank.

    Each ml of 0.1M NaNO₂ is equivalent to 0.02533gm of C₁₀H₁₁C₁N₃O₃S

Percentage purity: 

Report: The percentage purity of Sulphamethoxazole was found to be____%