Assay of Chlorpromazine

 

Aim: To perform the Assay of Chlorpromazine.

Apparatus: 

• Erlenmeyer flask.
• Volumetric flask.
•  Pipette
• Burette.

Chemicals Required: 

• Perchloric acid(0.1M)
• Chlorpromazine.
• Mercuric acetate solution(5%w/v in acetic acid).
• Crystal violet solution(0.2%w/v in acetic acid), 
• Acetone.
• methyl orange indiactoe,
• Potassium hydrogen phthalate.
• glacial acetic acid.
• Crystal violet indiactor

Principle:

Chlorpromazine is estimated by non-aqueous titration which is suitable for titration of weak acid and weak base. In this non aqueous solvent like perchloric acid is utilized as a titrant and methyl orange is used as an indicator. Mercuric acetate is added in the non-aqueous titration in order to remove the chloride ions. So, as to prevent the interference of the chloride ion released by the titrant. The mercuric acetate replaces the haladie ion in chlorpromazine with acetate ion which is a strong base. The end point is indicated by appearance of blue color.

Procedure: 

a) Standardisation of Percholoric acid(0.1N) 

Dissolved 0.5g of potassium hydrogen phthalate in 25ml of glacial acetic acid added few drops of 5%w/v crystal violet indicator. Titrated the solution with 0.1N perchloric acid till blue green color appears.

Sl no	Burettte reading		Volume consumed	Average
	Initial	Final

Molarity= 

b) Assay of Chlorpromazine hydrochloride

Accurately about 0.6 gm was weighed out, dissolved in 200 ml of acetone and 15 ml of
mercuric acetate solution was added to it. Titration was carried out with 0.1 M perchloric
acid, using a saturated solution of methyl orange in acetone as indicator. A blank titration was
also carried out.
1 ml of 0.1 M perchloric acid is equivalent to 0.03553 g of C₁₇H₁₉ClN₂S, HCl. 

% purity of Chlorpromazine hydrochloride =

Sl no	Burettte reading		Volume consumed
	Initial	Final

Report: The given sample Contains _______mg of chlorpromazine