Aim: To perform the Assay of Atropine.
Apparatus:
- Erlenmeyer flask.
- Volumetric flask.
- pipette,
- Burette.
Chemicals Required:
- Perchloric acid(0.1M)
- Atropine
- Glacial acetic acid.
- Crystal Violet solution(0.2%w/v in acetic acid)
- Acetone
- methyl orange indicator.
Principle: Atropine is assayed by non-aqueous titration which is generally used for the titration of weak acid with weak base. In this titration non-aqueous solvent perchloric acid is used and crystal violet is used as an indicator. At the end point blue colour is obtained.
Procedure:
a) Standardisation of perchloric acid (0.1N)
Dissolved 0.5g of potassium hydrogen phthalkate in 25ml of glacial acetic acid and few drops of 55 w/v crystal violet indicator. Titrated the solution with 0.1N perchloric acid till blue green colour appears.
| Sl no | Burettte reading | Volume consumed | |
|---|---|---|---|
| Initial | Final | ||
Molarity
b) Assay of Atropine:
Weighed accuretly 400mg of atropine and dissolved it in 50ml of glacial acetic acid and added a drop of crystal violet indicator. Titrated this solution with 0.1N perchloric acid until green color is obtained end point.
%purity of Atropine =
Report: The percentage purity of Atropine was found to be ____%
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